Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology

The ubiquitous members of the multicopper oxidase family of enzymes oxidize a range of aromatic substrates such as polyphenols, methoxy-substituted phenols, amines and inorganic compounds, concomitantly with the reduction of molecular dioxygen to water. This family of enzymes can be broadly divided into two functional classes: metalloxidases and laccases. Several prokaryotic metalloxidases have been described in the last decade showing a robust activity towards metals, such as Cu(I), Fe(II) or Mn(II) and have been implicated in the metal metabolism of the corresponding microorganisms. Many laccases, with a superior efficiency for oxidation of organic compounds when compared with metals, have also been identified and characterized from prokaryotes, playing roles that more closely conform to those of intermediary metabolism. This review aims to present an update of current knowledge on prokaryotic multicopper oxidases, with a special emphasis on laccases, anticipating their enormous potential for industrial and environmental applications.     

粒子的散射方程、孤子解和高能统计模型

基于粒子散射的实验结果和已知的理论,首先,讨论了散射的各种方程,包括Lipkin方程、Pearson方程和Laguerre方程等,并且应用于各种散射;其次,重点研究了非线性方程,如Kd V方程、非线性统一方程等及其孤子解;然后,探讨了共振态和弹性、非弹性碰撞;进而,讨论高能散射和相应的统计模型;最后,提出不同能量的散射可能对应粒子低能具有对称性和高能具有统计性的新二重性.     

等候区手术患者家属的心理影响因素及干预措施

目的:探讨等候区手术患者家属的心理影响因素及其干预措施.方法:通过临床护理实践及查阅相关文献进行总结分析.结果:影响因素及其干预措施包括详解病情及手术风险性;对手术环境及术中情况的告知;完善急诊手术患者家属心理干预;根据不同文化水平进行健康宣教;尽量以病情为主,设计最经济有效的治疗方案;提高医护人员从业技能及道德品质.结论:对等候区手术患者家属进行积极有效的心理干预,可很大程度上减轻患者家属的焦虑程度,缓和医患关系,一定程度上提升了医疗质量.     

智能下肢助力装置虚拟样机的联合仿真

尝试建立一种全新的智能助力装置虚拟样机模型,旨为提高下肢助力装置设计的效率与可靠性.将CATIA建立的机械模型导入ADAMS建立装置的三维虚拟样机模型,对助力装置的运动学及动力学进行了仿真验证,将虚拟样机模型与MATLAB/simulink结合,对助力装置动力学方程及搭建的控制系统进行联合仿真.根据人体的步态数据,采用PID控制算法对下肢助力系统进行控瓤仿真结果表明,下肢助力控制系统有较好的动态响应特性和轨迹跟踪能力.研究结果能及时发现装置存在的缺点与不足,为助力装置样机研制和电机选型提供了参考,也为实现装置的助力提供了重要的理论依据.     

基于应用驱动的编译原理教学研究

针对当前编译原理教学效率低下的问题,研究了基于应用驱动的编译原理课程教学方法.分析了理论教学与实验教学相融合的应用驱动教学基本原理与方法,对于每个知识点设计两个层次的题目,在第一层次题目的作用下,形成以教师为主导,以实验题目为主线,学生积极参与讨论的教学模式,增强授课效率,提高学生实验的自信心;通过第二层次题目,进行知识的巩固与强化,培养学生团队协作的能力.讨论了应用驱动教学法在编译原理教学中实施的具体措施以及应该重点注意的问题,这对于改善编译原理课程的教学现状,提高编译原理课程的教学效率具有重要的参考意义.     

财经类高校大学生应用科技能力培养新模式

近年来,随着社会经济的不断快速发展,社会对于人才的需求也有了更高的要求.针对当前财经类人才的需求与供给之间,市场的大量需求与财经类人才就业难同时存在等愈加明显的矛盾,该文就当前财经类高校本科教育中所存在的不足和弊端,以国内外研究为基础,针对学生应用科技能力的培养,提出了"两个结合、三个模块"和"五个方向"的"2+3+5"组织教学模式体系,并对金融工程专业学生实施了实证教学分析和调查研究.在"2+3+5"培养模式下,金融工程专业学生的创新、应用科技能力以及综合素质均得到了较大的提高.     

Synergetic effect of multiple phases on hydrogen desorption kinetics and cycle durability in ball milled MgH2–PrF3–Al–Ni composite

A new MgH₂–PrF₃–Al–Ni composite was prepared by ball milling under hydrogen atmosphere. After initial dehydrogenation and rehydrogenation, Pr₃Al₁₁, MgF₂, PrH₃ and Mg₂NiH₄ nanoparticles formed accompanying the main phase MgH₂. The hydrogen absorption-desorption properties were measured by using a Sieverts-type apparatus. The results showed that the MgH₂–PrF₃–Al–Ni composite improved cycle stability and enhanced hydrogen desorption kinetics. The improvement of hydrogen absorption-desorption properties is ascribed to the synergetic effect of the in situ formed Pr₃Al₁₁, MgF₂, PrH₃ and Mg₂NiH₄ nanoparticles. This work provides an important inspiration for the improvement of hydrogen storage properties in Mg-based materials.